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Science Cafe
Meta-transcriptomic Analysis Pipeline
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Step 1
Transformation between FASTQ and FASTA formatPerl Script: #1
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Step 2
Data preprocessing of the original sequence dataPerl Script: #1, #2
- Step 3 Perl Script: #1, #2, #3
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Step 4
BLASTN search against Silva databasePerl Script: #1, #2, #3, #4, #5, #6
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Step 5
Taxonomic assignment of rRNAsPerl Script: #1
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Step 6
BLASTX search against NCBI nr databasePerl Script: #1, #2, #3
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Step 7
Summary of expressed genes (sum of matched reads, % similarity, nr and SEED annotation)
